diff --git a/src/dawn_native/d3d12/D3D12Backend.cpp b/src/dawn_native/d3d12/D3D12Backend.cpp
index e6ce0f6..c0cbe48 100644
--- a/src/dawn_native/d3d12/D3D12Backend.cpp
+++ b/src/dawn_native/d3d12/D3D12Backend.cpp
@@ -108,9 +108,7 @@
 
         Ref<TextureBase> texture = backendDevice->CreateExternalTexture(
             &textureDescriptor, mD3D12Resource, std::move(d3d11on12Resource),
-            ExternalMutexSerial(descriptor->acquireMutexKey),
-            ExternalMutexSerial(descriptor->releaseMutexKey), descriptor->isSwapChainTexture,
-            descriptor->isInitialized);
+            descriptor->isSwapChainTexture, descriptor->isInitialized);
 
         return ToAPI(texture.Detach());
     }
diff --git a/src/dawn_native/d3d12/DeviceD3D12.cpp b/src/dawn_native/d3d12/DeviceD3D12.cpp
index 7dd2a53..54c2a71 100644
--- a/src/dawn_native/d3d12/DeviceD3D12.cpp
+++ b/src/dawn_native/d3d12/DeviceD3D12.cpp
@@ -534,16 +534,13 @@
         const TextureDescriptor* descriptor,
         ComPtr<ID3D12Resource> d3d12Texture,
         Ref<D3D11on12ResourceCacheEntry> d3d11on12Resource,
-        ExternalMutexSerial acquireMutexKey,
-        ExternalMutexSerial releaseMutexKey,
         bool isSwapChainTexture,
         bool isInitialized) {
         Ref<Texture> dawnTexture;
-        if (ConsumedError(
-                Texture::CreateExternalImage(this, descriptor, std::move(d3d12Texture),
-                                             std::move(d3d11on12Resource), acquireMutexKey,
-                                             releaseMutexKey, isSwapChainTexture, isInitialized),
-                &dawnTexture)) {
+        if (ConsumedError(Texture::CreateExternalImage(this, descriptor, std::move(d3d12Texture),
+                                                       std::move(d3d11on12Resource),
+                                                       isSwapChainTexture, isInitialized),
+                          &dawnTexture)) {
             return nullptr;
         }
         return {dawnTexture};
diff --git a/src/dawn_native/d3d12/DeviceD3D12.h b/src/dawn_native/d3d12/DeviceD3D12.h
index 26851d7..64c0e35 100644
--- a/src/dawn_native/d3d12/DeviceD3D12.h
+++ b/src/dawn_native/d3d12/DeviceD3D12.h
@@ -131,8 +131,6 @@
         Ref<TextureBase> CreateExternalTexture(const TextureDescriptor* descriptor,
                                                ComPtr<ID3D12Resource> d3d12Texture,
                                                Ref<D3D11on12ResourceCacheEntry> d3d11on12Resource,
-                                               ExternalMutexSerial acquireMutexKey,
-                                               ExternalMutexSerial releaseMutexKey,
                                                bool isSwapChainTexture,
                                                bool isInitialized);
 
diff --git a/src/dawn_native/d3d12/IntegerTypes.h b/src/dawn_native/d3d12/IntegerTypes.h
index df00f1e..b2f3309 100644
--- a/src/dawn_native/d3d12/IntegerTypes.h
+++ b/src/dawn_native/d3d12/IntegerTypes.h
@@ -26,9 +26,6 @@
     // BindGroup allocations.
     using HeapVersionID = TypedInteger<struct HeapVersionIDT, uint64_t>;
 
-    // The monotonically increasing serial for external D3D12 mutexes imported in Dawn.
-    using ExternalMutexSerial = TypedInteger<struct ExternalMutexSerialT, uint64_t>;
-
 }  // namespace dawn_native::d3d12
 
 #endif  // DAWNNATIVE_D3D12_INTEGERTYPES_H_
diff --git a/src/dawn_native/d3d12/TextureD3D12.cpp b/src/dawn_native/d3d12/TextureD3D12.cpp
index 3c8178f..bf07cad 100644
--- a/src/dawn_native/d3d12/TextureD3D12.cpp
+++ b/src/dawn_native/d3d12/TextureD3D12.cpp
@@ -34,6 +34,7 @@
 namespace dawn_native::d3d12 {
 
     namespace {
+
         D3D12_RESOURCE_STATES D3D12TextureUsage(wgpu::TextureUsage usage, const Format& format) {
             D3D12_RESOURCE_STATES resourceState = D3D12_RESOURCE_STATE_COMMON;
 
@@ -517,15 +518,12 @@
         const TextureDescriptor* descriptor,
         ComPtr<ID3D12Resource> d3d12Texture,
         Ref<D3D11on12ResourceCacheEntry> d3d11on12Resource,
-        ExternalMutexSerial acquireMutexKey,
-        ExternalMutexSerial releaseMutexKey,
         bool isSwapChainTexture,
         bool isInitialized) {
         Ref<Texture> dawnTexture =
             AcquireRef(new Texture(device, descriptor, TextureState::OwnedExternal));
         DAWN_TRY(dawnTexture->InitializeAsExternalTexture(
-            descriptor, std::move(d3d12Texture), std::move(d3d11on12Resource), acquireMutexKey,
-            releaseMutexKey, isSwapChainTexture));
+            descriptor, std::move(d3d12Texture), std::move(d3d11on12Resource), isSwapChainTexture));
 
         // Importing a multi-planar format must be initialized. This is required because
         // a shared multi-planar format cannot be initialized by Dawn.
@@ -553,15 +551,11 @@
         const TextureDescriptor* descriptor,
         ComPtr<ID3D12Resource> d3d12Texture,
         Ref<D3D11on12ResourceCacheEntry> d3d11on12Resource,
-        ExternalMutexSerial acquireMutexKey,
-        ExternalMutexSerial releaseMutexKey,
         bool isSwapChainTexture) {
         DAWN_TRY(CheckHRESULT(d3d11on12Resource->GetDXGIKeyedMutex()->AcquireSync(
-                                  uint64_t(acquireMutexKey), INFINITE),
+                                  kDXGIKeyedMutexAcquireReleaseKey, INFINITE),
                               "D3D12 acquiring shared mutex"));
 
-        mAcquireMutexKey = acquireMutexKey;
-        mReleaseMutexKey = releaseMutexKey;
         mD3D11on12Resource = std::move(d3d11on12Resource);
         mSwapChainTexture = isSwapChainTexture;
 
@@ -680,7 +674,7 @@
         mSwapChainTexture = false;
 
         if (mD3D11on12Resource != nullptr) {
-            mD3D11on12Resource->GetDXGIKeyedMutex()->ReleaseSync(uint64_t(mReleaseMutexKey));
+            mD3D11on12Resource->GetDXGIKeyedMutex()->ReleaseSync(kDXGIKeyedMutexAcquireReleaseKey);
         }
     }
 
diff --git a/src/dawn_native/d3d12/TextureD3D12.h b/src/dawn_native/d3d12/TextureD3D12.h
index 569c355..fc08940 100644
--- a/src/dawn_native/d3d12/TextureD3D12.h
+++ b/src/dawn_native/d3d12/TextureD3D12.h
@@ -45,8 +45,6 @@
             const TextureDescriptor* descriptor,
             ComPtr<ID3D12Resource> d3d12Texture,
             Ref<D3D11on12ResourceCacheEntry> d3d11on12Resource,
-            ExternalMutexSerial acquireMutexKey,
-            ExternalMutexSerial releaseMutexKey,
             bool isSwapChainTexture,
             bool isInitialized);
         static ResultOrError<Ref<Texture>> Create(Device* device,
@@ -96,8 +94,6 @@
         MaybeError InitializeAsExternalTexture(const TextureDescriptor* descriptor,
                                                ComPtr<ID3D12Resource> d3d12Texture,
                                                Ref<D3D11on12ResourceCacheEntry> d3d11on12Resource,
-                                               ExternalMutexSerial acquireMutexKey,
-                                               ExternalMutexSerial releaseMutexKey,
                                                bool isSwapChainTexture);
         MaybeError InitializeAsSwapChainTexture(ComPtr<ID3D12Resource> d3d12Texture);
 
@@ -136,8 +132,6 @@
         bool mSwapChainTexture = false;
         D3D12_RESOURCE_FLAGS mD3D12ResourceFlags;
 
-        ExternalMutexSerial mAcquireMutexKey = ExternalMutexSerial(0);
-        ExternalMutexSerial mReleaseMutexKey = ExternalMutexSerial(0);
         Ref<D3D11on12ResourceCacheEntry> mD3D11on12Resource;
     };
 
diff --git a/src/include/dawn_native/D3D12Backend.h b/src/include/dawn_native/D3D12Backend.h
index 8973af1..d614b16 100644
--- a/src/include/dawn_native/D3D12Backend.h
+++ b/src/include/dawn_native/D3D12Backend.h
@@ -55,9 +55,14 @@
         HANDLE sharedHandle;
     };
 
+    // Keyed mutex acquire/release uses a fixed key of 0 to match Chromium behavior.
+    constexpr UINT64 kDXGIKeyedMutexAcquireReleaseKey = 0;
+
     struct DAWN_NATIVE_EXPORT ExternalImageAccessDescriptorDXGIKeyedMutex
         : ExternalImageAccessDescriptor {
       public:
+        // TODO(chromium:1241533): Remove deprecated keyed mutex params after removing associated
+        // code from Chromium - we use a fixed key of 0 for acquire and release everywhere now.
         uint64_t acquireMutexKey;
         uint64_t releaseMutexKey;
         bool isSwapChainTexture = false;
diff --git a/src/tests/end2end/D3D12ResourceWrappingTests.cpp b/src/tests/end2end/D3D12ResourceWrappingTests.cpp
index 34f7a58..f12e98c 100644
--- a/src/tests/end2end/D3D12ResourceWrappingTests.cpp
+++ b/src/tests/end2end/D3D12ResourceWrappingTests.cpp
@@ -27,6 +27,8 @@
 
 namespace {
 
+    using dawn_native::d3d12::kDXGIKeyedMutexAcquireReleaseKey;
+
     class D3D12ResourceTestBase : public DawnTest {
       protected:
         std::vector<wgpu::FeatureName> GetRequiredFeatures() override {
@@ -126,8 +128,6 @@
             }
 
             dawn_native::d3d12::ExternalImageAccessDescriptorDXGIKeyedMutex externalAccessDesc;
-            externalAccessDesc.acquireMutexKey = 0;
-            externalAccessDesc.releaseMutexKey = 1;
             externalAccessDesc.usage = static_cast<WGPUTextureUsageFlags>(dawnDesc->usage);
 
             *dawnTexture = wgpu::Texture::Acquire(
@@ -366,7 +366,7 @@
         hr = mD3d11Device->CreateRenderTargetView(d3d11Texture.Get(), nullptr, &d3d11RTV);
         ASSERT_EQ(hr, S_OK);
 
-        hr = dxgiKeyedMutex->AcquireSync(0, INFINITE);
+        hr = dxgiKeyedMutex->AcquireSync(kDXGIKeyedMutexAcquireReleaseKey, INFINITE);
         ASSERT_EQ(hr, S_OK);
 
         const float colorRGBA[] = {
@@ -374,7 +374,7 @@
             static_cast<float>(clearColor.b), static_cast<float>(clearColor.a)};
         mD3d11DeviceContext->ClearRenderTargetView(d3d11RTV.Get(), colorRGBA);
 
-        hr = dxgiKeyedMutex->ReleaseSync(1);
+        hr = dxgiKeyedMutex->ReleaseSync(kDXGIKeyedMutexAcquireReleaseKey);
         ASSERT_EQ(hr, S_OK);
 
         dawn_native::d3d12::ExternalImageDescriptorDXGISharedHandle externalImageDesc = {};
@@ -386,8 +386,6 @@
             dawn_native::d3d12::ExternalImageDXGI::Create(device.Get(), &externalImageDesc);
 
         dawn_native::d3d12::ExternalImageAccessDescriptorDXGIKeyedMutex externalAccessDesc;
-        externalAccessDesc.acquireMutexKey = 1;
-        externalAccessDesc.releaseMutexKey = 2;
         externalAccessDesc.isInitialized = isInitialized;
         externalAccessDesc.usage = static_cast<WGPUTextureUsageFlags>(dawnDescriptor->usage);
 
@@ -397,11 +395,10 @@
         *dxgiKeyedMutexOut = dxgiKeyedMutex.Detach();
     }
 
-    void ExpectPixelRGBA8EQ(UINT64 acquireKey,
-                            ID3D11Texture2D* d3d11Texture,
+    void ExpectPixelRGBA8EQ(ID3D11Texture2D* d3d11Texture,
                             IDXGIKeyedMutex* dxgiKeyedMutex,
                             const wgpu::Color& color) {
-        HRESULT hr = dxgiKeyedMutex->AcquireSync(acquireKey, INFINITE);
+        HRESULT hr = dxgiKeyedMutex->AcquireSync(kDXGIKeyedMutexAcquireReleaseKey, INFINITE);
         ASSERT_EQ(hr, S_OK);
 
         D3D11_TEXTURE2D_DESC texture2DDesc;
@@ -451,7 +448,7 @@
 
         mD3d11DeviceContext->Unmap(spD3DTextureStaging.Get(), 0);
 
-        hr = dxgiKeyedMutex->ReleaseSync(acquireKey + 1);
+        hr = dxgiKeyedMutex->ReleaseSync(kDXGIKeyedMutexAcquireReleaseKey);
         ASSERT_EQ(hr, S_OK);
     }
 };
@@ -527,7 +524,7 @@
     // Now that Dawn (via D3D12) has finished writing to the texture, we should be
     // able to read it back by copying it to a staging texture and verifying the
     // color matches the D3D12 clear color.
-    ExpectPixelRGBA8EQ(2, d3d11Texture.Get(), dxgiKeyedMutex.Get(), d3d12ClearColor);
+    ExpectPixelRGBA8EQ(d3d11Texture.Get(), dxgiKeyedMutex.Get(), d3d12ClearColor);
 }
 
 // 1. Create and clear a D3D11 texture
@@ -560,7 +557,7 @@
     // Now that Dawn (via D3D12) has finished writing to the texture, we should be
     // able to read it back by copying it to a staging texture and verifying the
     // color matches the last D3D12 clear color.
-    ExpectPixelRGBA8EQ(2, d3d11Texture.Get(), dxgiKeyedMutex.Get(), d3d12ClearColor2);
+    ExpectPixelRGBA8EQ(d3d11Texture.Get(), dxgiKeyedMutex.Get(), d3d12ClearColor2);
 }
 
 // 1. Create and clear a D3D11 texture with clearColor
@@ -608,8 +605,6 @@
 
     // Create another Dawn texture then clear it with another color.
     dawn_native::d3d12::ExternalImageAccessDescriptorDXGIKeyedMutex externalAccessDesc;
-    externalAccessDesc.acquireMutexKey = 1;
-    externalAccessDesc.releaseMutexKey = 2;
     externalAccessDesc.isInitialized = true;
     externalAccessDesc.usage = static_cast<WGPUTextureUsageFlags>(baseDawnDescriptor.usage);
 
@@ -634,8 +629,6 @@
     DAWN_TEST_UNSUPPORTED_IF(UsesWire());
 
     dawn_native::d3d12::ExternalImageAccessDescriptorDXGIKeyedMutex externalAccessDesc;
-    externalAccessDesc.acquireMutexKey = 1;
-    externalAccessDesc.releaseMutexKey = 2;
     externalAccessDesc.isInitialized = true;
 
     wgpu::Texture texture;
@@ -678,8 +671,6 @@
 
     // Create the Dawn texture then clear it to blue using the second device.
     dawn_native::d3d12::ExternalImageAccessDescriptorDXGIKeyedMutex externalAccessDesc;
-    externalAccessDesc.acquireMutexKey = 1;
-    externalAccessDesc.releaseMutexKey = 2;
     externalAccessDesc.usage = static_cast<WGPUTextureUsageFlags>(baseDawnDescriptor.usage);
 
     wgpu::Device otherDevice = wgpu::Device::Acquire(GetAdapter().CreateDevice());
@@ -694,7 +685,6 @@
     otherTexture.Destroy();
 
     // Re-create the Dawn texture using the first (default) device.
-    externalAccessDesc.acquireMutexKey = 2;
     externalAccessDesc.isInitialized = true;
     texture =
         wgpu::Texture::Acquire(externalImage->ProduceTexture(device.Get(), &externalAccessDesc));
diff --git a/src/tests/end2end/VideoViewsTests_win.cpp b/src/tests/end2end/VideoViewsTests_win.cpp
index 9439609..18b63a0 100644
--- a/src/tests/end2end/VideoViewsTests_win.cpp
+++ b/src/tests/end2end/VideoViewsTests_win.cpp
@@ -144,10 +144,11 @@
         hr = d3d11Texture.As(&dxgiKeyedMutex);
         ASSERT(hr == S_OK);
 
-        hr = dxgiKeyedMutex->AcquireSync(0, INFINITE);
+        using dawn_native::d3d12::kDXGIKeyedMutexAcquireReleaseKey;
+        hr = dxgiKeyedMutex->AcquireSync(kDXGIKeyedMutexAcquireReleaseKey, INFINITE);
         ASSERT(hr == S_OK);
 
-        hr = dxgiKeyedMutex->ReleaseSync(1);
+        hr = dxgiKeyedMutex->ReleaseSync(kDXGIKeyedMutexAcquireReleaseKey);
         ASSERT(hr == S_OK);
 
         // Open the DX11 texture in Dawn from the shared handle and return it as a WebGPU
@@ -164,8 +165,6 @@
         ::CloseHandle(sharedHandle);
 
         dawn_native::d3d12::ExternalImageAccessDescriptorDXGIKeyedMutex externalAccessDesc;
-        externalAccessDesc.acquireMutexKey = 1;
-        externalAccessDesc.releaseMutexKey = 2;
         externalAccessDesc.isInitialized = true;
         externalAccessDesc.usage = static_cast<WGPUTextureUsageFlags>(textureDesc.usage);